Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury Intro:
Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury Abstract: Human ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50–100 nm. Here we show
that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and
were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained
coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous
population of particles with a hydrodynamic radius of 55–65 nm by size-exclusion fractionation on a HPLC. Together these
observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of
myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes.
This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury
and repair, and engenders novel approaches to the development of biologics for tissue repair.
Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury – Full Study
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